Identification of Seedborne Fungi on Soybean (Glycine max L.) Seeds Grown in Mediterranean Region of Turkey

Soybean (Glycine max L.) is one of the most valuable oilseed crops in world. It not only an oil seed crop and feed for livestock, but also mineral vitamins sources human diet. The soybean yield affected by various biotic abiotic stress factors all growing seasons. Diseases are significant that reduce growth yield. Fungi important pathogens affecting quality attacking plants during period after harvest. This study was conducted to detect identify seed-borne fungi associated with seed. From this context, 150 seeds were randomly chosen from experimental fields Akdeniz University Antalya province Turkey. These sterilized 70% ethanol 1 min, followed 10% sodium hypochlorite min then rinsed sterile water placed Petri plates using agar plate method. A total four species namely Aspergillus spp., Penicillium Cladosporium spp. Fusarium isolated seeds. Additionally, Genomic DNAs these fungal extracted internal transcribed spacer (ITS) region ribosomal DNA amplified ITS-1 ITS-4 primers a thermal cycler. After sequencing products, sequences aligned. BLASTn analysis each sequence showed had similarity (99%) isolates deposited GenBank.